Flow cytometry protocol for thp-1 cell
WebLearn about controls for flow cytometry including isotype controls, Fc blocks, FMO controls, live-dead, unstained controls, compensation controls and biological controls for surface & intracellular staining. ... WebOct 1, 2024 · Cell yields resulting from THP-1 differentiation in plates (pDMs) and differentiation in tubes (tDMs) were compared by flow cytometry. Cells were …
Flow cytometry protocol for thp-1 cell
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WebPlease refer to the product webpage and product-specific protocol to determine whether it is compatible with live cell staining. Collect cells by centrifugation and aspirate supernatant. Resuspend cells in 0.5–1 ml 1X PBS. Add formaldehyde to obtain a final concentration of 4%. Fix for 15 min at room temperature. WebJun 1, 2024 · This protocol provides instructions to perform a high-throughput small molecule screen using flow cytometry analysis of THP-1 cells, a human monocytic leukemia cell line. We describe a …
WebCommon cell preparation technique and staining protocols for surface and trapped markers to help get the best quality flow cytometry data. Locate out more. Standard per preparation techniques and coloring protocols for emerge press intracellular markers to assist get the best quality run cytometry data. Find out more WebCell cycle assay protocols for flow cytometry. Vybrant DyeCycle Violet Stain. Vybrant DyeCycle Green and Orange Stains. Vybrant DyeCycle Ruby Stain. FxCycle Violet …
WebFlow Cytometry Blocking Controls Fc receptors are found on monocytes, macrophages, dendritic cells and B cells. ... have been developed which, when added to a staining protocol, can ensure that only antigen specific … WebSep 1, 2015 · THP-1, a human monocytic cell line and its derived macrophages serve as a useful cell culture model for mechanistic studies of hCMV infection and its underlying biology. A major methodological challenge is the lack of a quick and reliable tool to accurately determine the efficiency of hCMV infection in THP-1 derived macrophages.
WebIntracellular Staining Flow Cytometry Output Using Detergents to Permeabilize Mobile; Centrifuge cells as by Step 3 and resuspend include appropriate volume in Flow Cytometry Coloring Buffer press buffer of choice so that the final cellular concentration is 1 x 10 7 cells/mL (other cell concentrations may been reasonably for different experiments)
WebJun 5, 2015 · At 24 hpi, IE2-EYFP positive cells were measured by flow cytometry; (b) THP-1 monocytes were differentiated to THP-1 derived macrophages by the addition of PMA for 48 h followed by infection of the obtained differentiated THP-1 cells with TB40E/IE2-EYFP at an MOI of 0.1. At 24 hpi, IE2-positive cells were detected by flow … philips f30t8WebFeb 3, 2024 · Single-cell preparation from different organs, staining of cells with fluorophore-conjugated antibodies, and analysis of the stained cells using flow cytometry are as described 51. Briefly, cells ... philips f30t8 cwWebFor the detection of the viability of Jurkat and THP-1 cells, an MTT assay was employed as previously described . Briefly, both Jurkat and THP-1 cells in the logarithmic growth phase were seeded at a density of 1 × 10 5 cells/well on a 24-well plate and treated with various concentrations (40, 20, 10, 5 and 2.5 µM) of each compound. After the ... philips f30t8/cw 30 watt altoWebWith the same method, the IL-1β in THP-1 cells stimulated with 10 ng/mL of S. enteritidis LPS was 34.8%, whereas after selonsertib administration it was detected at 2.0% using flow cytometry (Table 1 and Figure 2). philips f30t8/cw 30 wattWebProtocol for high-throughput compound screening using flow cytometry in THP-1 cells. Flow cytometry is a valuable method for analyzing protein expressions at the single cell level but can be ... philips f30t8/cwWebApr 13, 2024 · The recent demonstration of TPI in flow cytometry (TPI-FC) condition 36,37,38 promises to bridge gaps with respect to the conventional imaging flow cytometry systems, i.e. allowing comparable ... truth finders phone number toll freeWebApr 14, 2024 · A similar approach has been used to characterize the adherence and transmigration properties of peripheral blood mononuclear cells on human aortic endothelial cells and brain microvascular endothelial cells . One limitation of the flow cytometry-based assay was the inherent variability in the cells used; for example, the passage number of … truth finder website