Flp-in-293
WebMar 22, 2024 · We then compared the BioID results of AURKB, BET1, CDK7, CDK8, GSK3B and RAB5A from the Flp-In T-REx 293 (Fig. 4c and Supplementary Data 1c), with the U-2 OS, DU-145 and Flp-In T-REx 293 generated ... WebZeocin resistant clonal cells contain a single stably integrated FRT site. Use with the Flp-In system for rapid generation of cell line expressing protein of interest. Tissues: Kidney: …
Flp-in-293
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WebAs shown in Fig. 2A, 2-EH caused an increase in intracellular Ca 2+ levels in both Flp-In 293/ hTRPA1 and Flp-In 293/mTRPA1 cells in a concentration-dependent manner (EC 50 values of 225 µM and ... WebThermo Fisher flp in 293 hek293frt cells Flp In 293 Hek293frt Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
WebJun 13, 2016 · The cytotoxicity test demonstrated that IC50 value of atorvastatin to inhibit the growth and/or viability of Flp-In-293/ABCB1 (2677G) cells was 5.44 ± 0.10 mM, which was significantly lower than those in Flp-In-293/ABCB1 (2677 T) (6.02 ± 0.07 mM) and Flp-In-293/ABCB1 (2677A) cells (5.95 ± 0.08 mM). ... WebArticle Snippet: The plasmid containing this locus was then transfected into HEK-293 TRex Flp-In cells (ThermoFisher) along with a pOG44 plasmid encoding a Flp-recombinase using Turbofectin according to manufacturer’s protocols. Techniques: Activation Assay, Fluorescence, Transfection.
WebFlp-In™-293 Human embryonic kidney 1 × 107 cells, frozen R750–07 Flp-In™-CV-1 African Green Monkey kidney 1 × 107 cells, frozen R752–07 Flp-In™-CHO Chinese Hamster … WebFlp-In™ 293 细胞系设计用于快速生成稳定细胞系,确保从 Flp-In™ 表达载体中高水平表达您的目标蛋白。这些细胞在转录活性基因组基因座处包含一个稳定整合的 FRT 位点 …
WebNov 1, 2010 · Wild-type and mutant BCRP-expressing HEK-293 or Flp-In-293 cells and the vector control cells were plated in four-chamber polysterene vessels on glass slides (Falcon; BD Biosciences, Bedford, MA) at ∼2 × 10 5 cells/well. Cells were grown to 80% confluence and washed twice with PBS at room temperature. Cells were then fixed with 4% ...
WebJan 6, 2024 · Firstly I inserted my target gene (pig calcium sensing receptor,pCaSR) into pcDNA 5/FRT vector, and then co-transfected pcDNA5/FRT/pCaSR and POG44 into Flp … react gitlabWebMethods: The human P-gp (ABCB1/Flp-In™-293) stable expression cells were established by using the Flp-In™ system. The effects of tenulin and isotenulin on cell viability were evaluated by SRB assays in established cell lines, sensitive cancer cell line (HeLaS3), and resistant cancer cell line (KB-vin). ... react glitch effectWebMay 1, 2008 · We expressed a total of seven non-synonymous SNP (single nucleotide polymorphism) variants in Flp-In-293 cells by using the Flp (flippase) recombinase system. Of these, ABCG2 F208S and S441N variants were found to be expressed at markedly low levels, whereas their mRNA levels were equal to those of the other SNP variants and … how to start hogsmeade questWebA useful addition to the Flp-In™ system (Flp-In™ T-Rex™) allows this expression to be controlled by the addition of a small molecule inducer to the cell culture medium. Stable cell lines generated as described here can be used to great advantage in the study of receptor pharmacology signalling and oligomerisation. less. react give props to childWebI am trying to generate stable cell line by using Flp-In 293 T-REx cells. I use 9:1 (w/w) ratio of pOG44:pcDNA-GOI and lipofectamine as a transfection reagent. how to start homeschooling in kansasWebMay 18, 2016 · Hek 293 cell line, Doxycycline, expression, tissue culture, cell culture, cancer, cell cycle checkpoint. react global custom eventsWebFlp-In™ 293 cell line is designed for rapid generation of stable cell lines that ensure high level expression of your protein of interest from a Flp-In™ expression vector. These cells … Flp-In™ 293 cell line is designed for rapid generation of stable cell lines that … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes how to start homeschooling in florida